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(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered)

State Icon Reaction EC-Number Confidence maximal confidence score from EnzymeDetector
(3R)-hydroxybutyryl-CoA + NADP+ <=> acetoacetyl-CoA + NADPH + H+ 1.1.1.100 3-oxoacyl-[acyl-carrier-protein] reductase
1.1.1.157 3-hydroxybutyryl-CoA dehydrogenase
1.1.1.36 acetoacetyl-CoA reductase



(3R)-hydroxybutyryl-CoA + H2O <=> (R)-3-hydroxybutanoate + CoA + H+ 3.1.2.2 palmitoyl-CoA hydrolase
3.1.2.20 acyl-CoA hydrolase
3.1.2.4 3-hydroxyisobutyryl-CoA hydrolase



(S)-3-hydroxybutyryl-CoA + NAD+ <=> acetoacetyl-CoA + NADH + H+ 1.1.1.178 3-hydroxy-2-methylbutyryl-CoA dehydrogenase
1.1.1.35 3-hydroxyacyl-CoA dehydrogenase
1.1.1.36 acetoacetyl-CoA reductase



(S)-3-hydroxybutyryl-CoA + H2O <=> L-3-hydroxybutyrate + CoA + H+ 3.1.2.4 3-hydroxyisobutyryl-CoA hydrolase

acetyl-CoA <=> acetoacetyl-CoA + CoA 1.1.1.34 hydroxymethylglutaryl-CoA reductase (NADPH)
2.3.1.155 acetyl-CoA C-myristoyltransferase
2.3.1.16 acetyl-CoA C-acyltransferase
2.3.1.38 [acyl-carrier-protein] S-acetyltransferase
2.3.1.9 acetyl-CoA C-acetyltransferase





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